First Report of Epicoccum tobaicum Associated with Leaf Spot on Flowering Cherry in South Korea

HomePlant DiseaseVol. 105, No. 9First Report of Epicoccum tobaicum Associated with Leaf Spot on Flowering Cherry in South Korea PreviousNext DISEASE NOTE OPENOpen Access licenseFirst KoreaV.-C. Han, N. H. Yu, Yoon, Y. K. Son, B.-H. Lee, and J.-C. KimV.-C. HanDepartment Agricultural Chemistry, Institute Environmentally Friendly Agriculture, College Agriculture Life Sciences, Chonnam National University, Buk-gu, Gwangju 61186, KoreaSearch for more papers by this author, YuDepartment YoonBiological Genetic Resources Assessment Division, Biological Resources, Seo-gu, Incheon 22689, SonBiological LeeBiological Kim†Corresponding author: Kim; E-mail Address: [email protected]://orcid.org/0000-0003-0753-1966Department author AffiliationsAuthors Affiliations V.-C. Han1 Yu1 Yoon2 Son2 Lee2 Kim1 † 1Department 2Biological Published Online:1 Oct 2021https://doi.org/10.1094/PDIS-12-20-2711-PDNAboutSectionsView articlePDFPDF PlusSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat View articleFlowering cherry (FC, Prunus × yedoensis Matsumura; Somei-yoshino cherry) is an ornamental tree, planted across producing stunning flowers spring. The seasonal blooms are annually celebrated during blossom festivals many locations the country. spot disease among most common important diseases affecting FC trees every year, resulting premature defoliation reduced flowering blossoms following year. In May 2018, brown spots (2 5 mm), circular irregular dark borders, were observed leaves Hadong, Gyeongsangnamdo, (35°07′48.9″N, 127°46′53.8″E), a incidence 55%. Single lesions often coalesced sometimes perforated, leaving shot holes. Sampled surface sterilized 1% NaOCl 1 min followed 70% ethanol 30 s then rinsed twice sterile distilled water. About 2-mm-long infected leaf pieces from margins put onto water agar (1.5% agar) plates incubated at 25°C 72 h. Mycelia grown symptomatic tissue transferred potato dextrose (PDA) plates, five similar fungal isolates obtained hyphal tips. They produced strong reddish-orange diffusible pigment PDA after days exudates 8 days. Conidia globular pear-shaped, dark, verrucose, multicellular, 14.8 23.5 μm diameter (average = 18.7 μm, n 30) isolate JCK-CSHF10. These morphological characteristics consistent genus. Three loci (ITS, tub2, rpb2) three (JCK-CSHF8, JCK-CSHF9, JCK-CSHF10) amplified using primer pairs ITS1F/LR5 (Gardes Bruns 1993; Vilgalys Hester 1990), Btub2Fd/Btub4Rd (Woudenberg et al. 2009), RPB2-5F2/RPB2-7cR (Liu 1999; Sung 2007), respectively. ITS, rpb2 sequences deposited GenBank (MW368668 MW368670, MW392083 MW392085, MW392086 MW392088, respectively), showing 99.6 100% identity layuense (E33), later synonym E. (Hou 2020). phylogenetic tree concatenated placed cluster (CBS 232.59, CGMCC 3.18362, CBS 384.36; Hou Taken together, identified as tobaicum. pathogenicity JCK-CSHF10 was tested 15 healthy (cv. Somei-yoshino, 1.2 m height) kept greenhouse. Five-millimeter-diameter plugs 7-day-old cultures or mycelia-free controls abaxial side points, previously wounded needle (Zlatković 2016). Inoculation sites covered moist cotton plugs. Trees clear plastic bag maintained high humidity darkness 24 h, 12-h photoperiod. Brown appeared inoculated 7 days, identical those field, whereas control remained symptomless. This experiment repeated times. A fungus same morphology recovered lesions, thus confirming Koch’s postulates. (syn. tobaicum) has been reported spot-causing agent Perilla sp. (Chen 2017) Camellia sinensis To date, there no report occurrence FC. our knowledge, first causing Korea.The author(s) declare conflict interest.References:Chen, Q., 2017. Stud. Mycol. 87:105. https://doi.org/10.1016/j.simyco.2017.06.002 Crossref, ISI, Google ScholarChen, J., 2020. Plant Dis. 104:2029. https://doi.org/10.1094/PDIS-09-19-1906-PDN Link, ScholarGardes, M., Bruns, T. D. 1993. Mol. Ecol. 2:113. https://doi.org/10.1111/j.1365-294X.1993.tb00005.x ScholarHou, L. W., 96:309. https://doi.org/10.1016/j.simyco.2020.05.001 ScholarLiu, 1999. Biol. Evol. 16:1799. https://doi.org/10.1093/oxfordjournals.molbev.a026092 ScholarSung, G. H., 2007. Phylogenet. 44:1204. https://doi.org/10.1016/j.ympev.2007.03.011 ScholarVilgalys, R., Hester, M. 1990. J. Bacteriol. 172:4238. https://doi.org/10.1128/JB.172.8.4238-4246.1990 ScholarWoudenberg, C., 2009. Persoonia 22:56. https://doi.org/10.3767/003158509X427808 ScholarZlatković, 2016. For. Pathol. 46:666. https://doi.org/10.1111/efp.12300 ScholarThe interest.Funding: research supported grant (NIBR), funded Ministry Environment (MOE) (NIBR202018201).DetailsFiguresLiterature CitedRelated Vol. 9 September 2021SubscribeISSN:0191-2917e-ISSN:1943-7692 DownloadCaptionKadsura coccinea caused Botryosphaeria dothidea (D. Su al.). Photo credit: Su. Ramularia coleosporii (M. Aktaruzzaman B. S. Kim. Injection maize plant classification standard stalk rot (W. Jiang Li. Metrics Downloaded 518 times Article History Issue Date: Dec 2021Published: 2021First Look: 18 Jan 2021Accepted: 17 2021 Page: 2734 Information© American Phytopathological SocietyFundingMinistry KoreaGrant/Award Number: NIBR202018201Keywordsflowering cherryleaf spotpathogenicityEpicoccum tobaicumThe interest.